Not known Facts About attune flow cytometer
Not known Facts About attune flow cytometer
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Info-enriched characterization of mobile populations. Morphological info from illustrations or photos can incorporate for the richness of flow cytometry details. For instance, the determine shows an or else common apoptosis assay utilizing Annexin V and PI, adding cell imaging to characterize cells in Each individual populace to reveal morphologically unique options. These insights couldn't have already been acquired from multiplex staining alone.
As samples are acquired around the Attune CytPix Flow Cytometer, the large-pace brightfield digicam captures and suppliers visuals of detected gatherings in a rate as much as 6,000 pictures for every second, according to the flow rate and picture sizing. For bigger adaptability, Attune Cytometric Software program lets you alter the image capture frequency as necessary and select a certain gate for impression seize, if demanded.
Combine acoustic concentrating know-how with significant-parameter Investigation for versatile spectral unmixing and standard workflows.
Explore new insights into your experimental details by visualizing populations using blended fluorescence and morphology information. Our software package interface wizards permit downsampling, concatenation (merged files) and dimensionality applications which can be created in the software program for an intuitive, streamlined information analysis expertise.
"A picture is just not really worthy of 1000 dots, but it really really really helps to see visuals. It is great to view images that correlate with staining. As an example, AnnexinV-optimistic cells that are morphologically unique than wholesome cells, or CD14+ monocytes that happen to be larger and more textured than CD3+/CD4+/CD14- lymphocytes.
Additionally, it is illuminating to find out how many doublets slip via regular singlet gates, and terrific to validate the position of unanticipated double-constructive gatherings in my analysis."
Pair your Attune flow cytometer using an autosampler for greater workflow performance, and add robotic integration for max throughput.
Regular picture high quality even at high throughput Acoustic focusing and a higher-velocity camera combine to picture these T cells continually at low or higher flow premiums. Easily regulate focus and digital camera configurations to meet experimental prerequisites.
Early signs of this contamination had originally been dismissed as particles. Backgating to find new populations. Backgating imaged cells around the Attune CytPix Flow Cytometer also lets you use morphological attributes to discover exciting subpopulations that would not be obvious from flow cytometry info by yourself.
Buyers can check out event photographs in true-time to verify sample top quality, optimize protocols, and file the morphology of each and every mobile inhabitants discovered for Evaluation. Imaging seize also Rewards from acoustic focusing, which helps to placement the cells to ensure that a pointy, centered image is obtained.
Audio waves enable exact cell alignment, causing Outstanding knowledge top quality and ~10X faster sample flow fees.
Software note: Automatic picture analysis lowers user-to-consumer variability in flow cytometry gating methods
Crafted-in dimensionality reduction tools amplified resolution into populations. Fluorescently gated populations back again-gated onto UMAP plot to verify anticipated inhabitants resolution in lysed whole human blood. User can look at gatherings from Every single cluster for Visible confirmation of mobile phenotypes.
No clean/no lyse leukocyte analysis with imaging. Cells had been obtained from 24-hour-aged blood by dilution in one mM EDTA ( Professional UX/UI allows consumers to teach AI styles for expanded applications. (A) The “Educate Model” aspect gives annotation tools and true-time design teaching precision.
Gating on these prolonged parameters enables you to quickly and correctly discover populations of desire and confirm gating system with little or no manual evaluate. The Attune computer software offers use of condition-of-the-artwork machine learning Assessment equipment in just an expertly created user interface, rendering it exceptionally user friendly.
As the mobile line had Earlier been analyzed within the Attune CytPix Flow Cytometer, the scientists went back again to the images and had been in a position to doc the microbial an infection at least five times earlier.
The Attune CytPix Flow Cytometer makes it possible for consumers to visualize samples through acquisition, verify the precision of gating methods, and support benefits with click here dependable documentation for publication applications.
What seemed to be a homogeneous inhabitants is actually more assorted—an Perception that needs to be regarded as when interpreting success.